enterotoxigenic e coli etec strain h10407  (ATCC)

Journal: Applied and Environmental Microbiology

Article Title: Presence and Characterization of Shiga Toxin-Producing Escherichia coli and Other Potentially Diarrheagenic E. coli Strains in Retail Meats ▿

doi: 10.1128/AEM.01968-09

Figure Lengend Snippet: Vero cell cytotoxicity of Shiga toxin-producing E. coli isolates from retail meats. The CD50 is expressed as the toxin dilution that causes 50% Vero cell detachment compared with untreated cells (control). The value on the y axis indicates the log of the reciprocal of the CD50. The data shown are averages for three independent assays. *, strains EDL933 and K-12 served as positive and negative controls, respectively.

Article Snippet: PCR products (10 μl) were examined in a 2% (wt/vol) agarose gel at 120 mV for 30 min. E. coli strains ATCC 35401 (containing elt and stI ), ATCC 43893 ( ipaH ), ATCC 43887 ( eae bfp ), 042 ( aafII ), and F1845 ( daaE ) were used as positive controls, while E. coli K-12 served as a negative control.

Techniques: Control

Journal:

Article Title: Real-Time Fluorescence PCR Assays for Detection and Characterization of Heat-Labile I and Heat-Stable I Enterotoxin Genes from Enterotoxigenic Escherichia coli

doi: 10.1128/JCM.42.9.4092-4100.2004

Figure Lengend Snippet: Amplification of LT and ST genes in separate LC-PCR assays. The graphs depict fluorescence at 640 and 705 nm versus cycle number for E. coli strains with various combinations of target genes. (A) LT genes. •, H10407 (LT I positive, ST Ia positive, ST Ib positive); □, F5176 (LT I positive, ST Ia negative, ST Ib negative); —, negative control. (B) ST genes. •, H10407 (LT I positive, ST Ia positive, ST Ib positive); ▪, TX1 (LT I negative, ST Ia negative, ST Ib positive); ×, C4046 (LT I negative, ST Ia negative, ST Ib positive); □, R554 (LT I negative, ST Ia negative, ST Ib positive); ○, F7682 (LT I negative, ST Ia positive, ST Ib negative); —, negative control.

Article Snippet: To assess the sensitivity of the PCR assays for the three target genes, E. coli H10407 strain ATCC 35401 was grown at 37°C in brain heart infusion broth (approximate optical density of 2.7 to 2.8 at 600 nm) and diluted 10-fold in phosphate-buffered saline to yield 10 1 to 10 10 CFU/ml as estimated by a standard plating procedure.

Techniques: Amplification, Fluorescence, Negative Control

Journal:

Article Title: Real-Time Fluorescence PCR Assays for Detection and Characterization of Heat-Labile I and Heat-Stable I Enterotoxin Genes from Enterotoxigenic Escherichia coli

doi: 10.1128/JCM.42.9.4092-4100.2004

Figure Lengend Snippet: (A) Melting curve analysis performed on amplification products of four different ST-positive isolates, representative of the different ST genotypes observed in this study. Strain H10407 represents a typical ST Ia-positive and ST Ib-positive isolate, strain F7682 is an isolate harboring a variant ST Ia sequence, and strains C4046 and R554 are ST Ib-positive isolates harboring variant ST Ib sequences. (B and C) Sequence alignments with amplicons of the four different ST-genotypes; the sequences under GenBank accession numbers M25607 (ST Ia), M34916 (ST Ib), and M18345 (variant ST Ib); and ST Ia-specific sensor hybridization probe ST-HP-1a (B), ST Ib-specific sensor hybridization probe ST-HP-1b (B), or anchor hybridization probe ST-HP-2 (C). Sequence identity is indicated by dashes. The observed Tms are indicated.

Article Snippet: To assess the sensitivity of the PCR assays for the three target genes, E. coli H10407 strain ATCC 35401 was grown at 37°C in brain heart infusion broth (approximate optical density of 2.7 to 2.8 at 600 nm) and diluted 10-fold in phosphate-buffered saline to yield 10 1 to 10 10 CFU/ml as estimated by a standard plating procedure.

Techniques: Amplification, Variant Assay, Sequencing, Hybridization

Journal:

Article Title: Real-Time Fluorescence PCR Assays for Detection and Characterization of Heat-Labile I and Heat-Stable I Enterotoxin Genes from Enterotoxigenic Escherichia coli

doi: 10.1128/JCM.42.9.4092-4100.2004

Figure Lengend Snippet: Distribution of T m s among 137 ETEC strains and associated sequence variation in the region of the hybridization probes

Article Snippet: To assess the sensitivity of the PCR assays for the three target genes, E. coli H10407 strain ATCC 35401 was grown at 37°C in brain heart infusion broth (approximate optical density of 2.7 to 2.8 at 600 nm) and diluted 10-fold in phosphate-buffered saline to yield 10 1 to 10 10 CFU/ml as estimated by a standard plating procedure.

Techniques: Sequencing, Hybridization

Journal:

Article Title: Real-Time Fluorescence PCR Assays for Detection and Characterization of Heat-Labile I and Heat-Stable I Enterotoxin Genes from Enterotoxigenic Escherichia coli

doi: 10.1128/JCM.42.9.4092-4100.2004

Figure Lengend Snippet: (A) Melting curve analysis performed on amplification products of two different LT-positive isolates. Strain H10407 represents a typical LT I-positive isolate, and strain F51761 is an ETEC isolate harboring a variant LT I sequence. (B and C) Sequence alignments with amplicons of the two different LT I-genotypes, the sequence under GenBank accession number S60731, and sensor hybridization probe LT-HP-1 (B) or anchor hybridization probe LT-HP-2 (C). Sequence identity is indicated by dashes. The observed Tms are indicated.

Article Snippet: To assess the sensitivity of the PCR assays for the three target genes, E. coli H10407 strain ATCC 35401 was grown at 37°C in brain heart infusion broth (approximate optical density of 2.7 to 2.8 at 600 nm) and diluted 10-fold in phosphate-buffered saline to yield 10 1 to 10 10 CFU/ml as estimated by a standard plating procedure.

Techniques: Amplification, Variant Assay, Sequencing, Hybridization